P-51 Epigenome-wide DNA methylation profiling identifies the TNXB gene as a potential epigenetic candidate for colorectal cancer prevention

Genetic and epigenetic alterations, as well environmental factors contribute to the colorectal carcinogenesis cancer progression. Particularly, aberrant DNA methylation is recognized a common molecular feature in human tumors. However, methylation-based biomarkers are still relatively new, potential required further validate them initiation progression for clinical use. We studied expression of TNXB gene healthy participants patients with CRC. evaluated full biomarker description value CRC, validated different biological tissues, assessed its role To test effect on expression, we treated HCT116 cells siRNA targeted gene. also determined effects cell proliferation, migration, key genes related tumorigenic properties. Here, study changes by genome-wide analysis tumors from (CRC). found total 73,509 differentially methylated CpG sites (DMCs) (false discovery rate (FDR) < 0.001) tumor area when compared NAT area. defined several signaling pathways that were epigenetically altered such focal adhesion, MAPK, PI3K, RAS pathways. discovered candidates be methylated, COL4A1, COL11A, ITGA4, OPLAH, MAD1L1, PRDM16, TNXB. Specifically, our filtered an epimutation Overall, hypomethylated (p 0.001), affect whole gene, including body promoter region. Furthermore, this was downregulated which adipose tissue, TCGA-COAD TCGA-READ cohorts. Functional system reported silencing increased proliferation overexpression inhibited stemness The TGFβ, suggesting link between regulation cycle through TGFβ. In summary, data identify Our observations suggest pathogenesis CRC influencing Therefore, targeting may novel mechanism interfere